 It was achieved 100% fertilizing brook trout - Salvelinus fontinalis М. eggs with semen from brown trout - Salmo trutta m. fario L. using of medium 49282 - single or along with extender 49283, but embryonic mortality was 100%.The ultimate variant /♂ Salvelinus fontinalis М. Х ♀ Salmo trutta m. fario L./ was successful for optimize crossbreeding.Medium 49282 have the best sperm activating effect and may use along with extender 49283 for artificial insemination to produce tiger trout.