 This points to a pioneering role for epsin in vesicle budding, as it provides both a driving force and a link between membrane invagination and clathrin polymerisation.In particular, epsin-1 shows specificity for the membrane glycophospholipid phosphatidylinositol-4,5-bisphosphate, however not all ENTH domains bind to this molecule.Binding causes tubulation of liposomes and "in vivo" this membrane-binding function is normally coordinated with clathrin polymerisation.