 As an alternative to punching out a paper disc, recent automation solutions extract the sample by flushing an eluent through the filter without punching it out.An automation including the application of an internal standard prior extraction was developed by the Swiss company CAMAG.The technology holds promise for expanding diagnostic services to HIV-infected infants in resource-poor settings due to the samples' longer lifespan with reduced need for refrigeration and the less invasive nature of the test compared with other methods.