 Probably, his most significant contribution was the development of a method of vaccination against anthrax.However, credit for creation of the anthrax vaccine went to Louis Pasteur, following Pasteur's celebrated demonstration with the vaccine on sheep at Pouilly-le-Fort in 1881.At Pouilly-le-Fort, Pasteur used a vaccine attenuated by potassium dichromate, employing a process similar to Toussaint's, who had published a means of attenuation by another antiseptic, carbolic acid.