 He died on 20 July 1646 and was buried in Westminster Abbey, but exhumed in 1661 and his remains deposited with those of dozens of other Parliamentarians in a pit in the churchyard of St Margaret's, Westminster.Twisse was a strong defender of a Calvinist, supralapsarian position.In his "Vindiciae gratiae" of 1632 he attacked Jacobus Arminius, and in "Dissertatio de scientia media" of 1639 adopted certain Dominican arguments, on justification.