 In-tube detection of LAMP DNA amplification is possible using manganese loaded calcein which starts fluorescing upon complexation of manganese by pyrophosphate during in vitro DNA synthesis.Another method for visual detection of the LAMP amplicons by the unaided eye was based on their ability to hybridize with complementary gold-bound ss-DNA and thus prevent the normal red to purple-blue color change that would otherwise occur during salt-induced aggregation of the gold particles.So, a LAMP method combined with amplicon detection by AuNP can have advantages over other methods in terms of reduced assay time, amplicon confirmation by hybridization and use of simpler equipment (i.e., no need for a thermocycler, electrophoresis equipment or a UV trans-illuminator.