 LAMP is an isothermal nucleic acid amplification technique.In contrast to the polymerase chain reaction (PCR) technology, in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature, and does not require a thermal cycler.In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C using either two or three sets of primers and a polymerase with high strand displacement activity in addition to a replication activity.