 This polysaccharide-coated lipid bilayer creates a permeability barrier that prevents efficient uptake of the compound.Addition of EDTA, which is known to strip the lipopolysaccharides and increase membrane permeability, removes the phloxine B resistance and allows gram-negative bacteria to be killed as well.Phloxine B can be used to stain dead cells of several yeasts, including "Saccharomyces cerevisiae" and "Schizosaccharomyces pombe".