 RNA-Seq transcriptomics by next gen sequencing will mean these barriers no longer hold true.Any organism's entire transcriptome could be potentially sequenced in one run (for very small bacterial genomes) and not only would the identification of each transcript be available but expression profiling is possible as quantitative reads can also be achieved.Chromatin immunoprecipitation (ChIP) is a method for determining transcription factor binding sites and DNA-protein interactions.