 Isaac Wake was the second son of Arthur, son of John Wake of Hartwell, Northamptonshire, a descendant of the lords of Blisworth.His father, a canon of Christ Church and master of St. John's Hospital in Northampton, was rector of Great Billing in Northamptonshire until 1573, when he was deprived for nonconformity; he afterwards lived for many years in Jersey.Born in 1580 or 1581, Isaac Wake entered Christ Church, Oxford in 1593, and graduated B.A.