 In 1558, he took his seat in the first Irish Parliament of Queen Elizabeth, but strongly opposed her "Act of Uniformity" of that year and for this and other actions, he was ordered to be arrested in 1567 and conveyed to London, but the order was not carried out.During the interval, however he had been commissioned as one of the Justices of the Peace for County Kildare during the temporary absence of the Lord Deputy in 1561.Roland Eustace married Joan, daughter of James Butler, 8th Baron Dunboyne in about 1528.