 Evidence for didactic organisation "in vivo" was first discovered through research into synaptic reorganisation in primary visual cortex that compared the results of neuronal recording experiments and computational models.However, the tendency of spike-timing-dependent plasticity to separate neurons into ‘teachers’ and ‘students’ had previously been predicted in theory based on computational modelling results alone.Didactic organisation is primarily a consequence of spike-timing-dependent plasticity, because when the neurons within an interconnected network undergo action potentials (or ‘spikes’) at approximately the same time (within the order of tens of milliseconds) the efferent synaptic connections of neurons that spike early will have their efficacy increased (long-term potentiation), while neurons that spike late will have the efficacy of their efferent synaptic connections decreased (long-term depression).