 HGF is the natural high-affinity ligand for Met.Its N-terminal region binds to Met and receptor dimerization as well as autophosphorylation of two tyrosines occur in the activation loop (A-loop) in the kinase domain of Met.Phosphorylation occurs in tyrosines close to the C-terminus, creating a multi-functional docking site
which recruits adaptor proteins and leads to downstream signalling.