 The S3-S4 link, where hanatoxin binds, is important for voltage-sensing and gate activation.By binding to the S3b segment, the S3b segment is pushed to the N-terminus of the S4 segment, restricting movement and, therefore, requiring a higher depolarization for channel-activation .While the effects of hanatoxin on its own are not thoroughly studied, it is part of the venom of "Grammostola spatulata", which is considered slightly venomous to humans.