 Then the depth-resolution information lacking in one stack is supplied from another stack; for example with two orthogonal stacks the (poor-resolution) axial direction in one stack is a (high-resolution) lateral direction in the other stack.The lateral resolution of LSFM can be improved beyond the Abbe limit, by using super resolution microscopy techniques, e.g.with using the fact, that single fluorophores can be located with much higher spatial precision than the nominal resolution of the used optical system (see stochastic localization microscopy techniques).