 The necrosome also causes leakage of lysosomal digestive enzymes into the cytoplasm by induction of reactive oxygen species by JNK, sphingosine production, and calpain activation by calcium release.Necroptosis has been implicated in the pathology of many types of acute tissue damage, including myocardinal infarction, stroke, ischemia-reperfusion injury.In addition, necroptosis is noted to contribute to atherosclerosis, pancreatitis, inflammatory bowel disease, neurodegeneration, and some cancers.