 The axial resolution is worse than the lateral (about a factor of 4), but it can be improved by using a thinner lightsheet in which case nearly isotropic resolution is possible.Thinner light sheets are either thin only in a small region (for Gaussian beams) or else specialized beam profiles such as Bessel beams must be used (besides added complexity, such schemes add side lobes which can be detrimental).Alternatively, isotropic resolution can be achieved by computationally combining 3D image stacks taken from the same sample under different angles.