 Enhanced concentration of calcium and phosphate ions in SBF solution accelerates the coating process and, in the meantime, eliminates the need for regular replenishment of the SBF solution.An attempt was made to investigate the application of SBF in gene delivery.Calcium phosphate nanoparticles, required for the delivery of plasmid DNA (pDNA) into the nucleus of the cells, were synthesized in a SBF solution and mixed with pDNA.