 Protein expression in levitated cultures shows striking similarity to in vivo patterns.N-cadherin expression in levitated human glioblastoma cells was identical to the expression seen in human tumor xenografts grown in immunodeficient mice, while standard 2D culture showed much weaker expression that did not match xenograft distribution as shown in the picture below.The transmembrane protein N-cadherin is often used as an indicator of in-vivo-like tissue assembly in 3D culturing.