 In addition to Sanger sequencing, Multiplex Ligation Probe Amplification (MLPA) can be used to determine if  duplications and deletions in  FOXC2 are present in an individual, making it a practical testing mechanism.Lastly, diagnosis is sometimes determined without genome testing.If an individual exhibits multiple symptoms of lymphedema-distichiasis and has a medical history consistent with known lymphedema-distichiasis symptoms, then their diagnosis is confirmed via clinical evaluation.