 Lieutenant Colonel Andrew W. Brown had no previous military experience.Major Lawrence D. Clark had served as captain of Co. A, 1st Vermont Infantry.Clark resigned in March 1863 because of impaired health, and was replaced by Captain Joseph J. Boynton, of Company C. Brown resigned in May 1863, and was succeeded by Captain William D. Munson, of Company D.

James Stevens Peck, originally a second lieutenant in Company I, was subsequently appointed regimental adjutant.