 And because no population derives all of its protein exclusively from a single food, the determination of BV of a single protein is of limited use for application to human protein requirements.Another limitation of the use of Biological Value as a measure of protein quality is that proteins which are completely devoid of one essential amino acid (EAA) can still have a BV of up to 40.This is because of the ability of organisms to conserve and recycle EAAs as an adaptation of inadequate intake of the amino acid.