 It is always the same whether at the first recognition site or the second in the following example sequence.For Sp1, which has multiple (16) sequence specificity as shown above, the two recognition sites in the following example sequence fragment are at 18 and 32, and their respective recognition sequences are 5'-GGGGCGGAGC-3' and 5'-TGGGCGGAAC-3'.5'-AACGTTAGCTGCAGTC"GGGGCGGAGC"TAGGCTGCAGGAAT"TGGGCGGAAC"CT-3'