 The transcription factor Sp1 for example, binds the sequences 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3', where (G/T) indicates that the domain will bind a guanine or thymine at this position.The restriction endonuclease PstI recognizes, binds, and cleaves the sequence 5'-CTGCAG-3'.A recognition sequence is different from a recognition site.