 The crucial issue in such experiments is to use probes that monitor different aspects of the structure.For example, DSC gives information on the heat capacity changes (and hence enthalpy) associated with unfolding, fluorescence on the immediate environment of the fluorophore, FRET on the average dimensions of the molecule and CD on the secondary structure.A more stringent test would involve following the chemical shifts of each and every atom in the molecule by nuclear magnetic resonance (NMR) as a function of temperature/denaturant.