 The mitotic spindle must also align parallel to the asymmetrically distributed cell fate determinants to allow them to become segregated into one daughter cell and not the other.The mitotic spindle orientation is mediated by Inscuteable, which is segregated to the apical cortex of the neuroblast.Without the presence of Inscuteable, the positioning of the mitotic spindle and the cell fate determinants in relationship to each other becomes randomized.