 Acquafresca returned to Bologna for the 2013–14 Serie A season; however, he made 19 appearances in Serie A without scoring a goal, while Bologna were relegated to Serie B at the end of the season.In the 2016–17 pre-season Acquafresca was left out from the first team squad, partially due to a trauma in his knee.He only played for Bologna as a member of the reserve team against the first team on 20 August, along with fellow first team player Luca Ceccarelli who was released by the club on 31 August.