 Incubation of the cell pack in a humid environment for time periods up to several days allows transient expression of protein.Secreted proteins can be washed out of the cell pack by application of buffer and further filtration.It's quite common to coinfiltrate the "Agrobacterium" carrying the construct of interest together with another "Agrobacterium" carrying a silencing suppressor protein gene such as the one encoding the p19 protein from the plant pathogenic Tomato bushy stunt virus (TBSV), or the NSs protein  from tomato spotted wilt virus (TSWV).