 Prence's will was dated March 13, 1672/73, proved June 5, 1673.He named his wife Mary, seven surviving daughters, Jane, the wife of Mark Snow; Mary Tracy; Sarah Howes; Elizabeth Howland; Judith Barker; Hannah; and Mercy; his grandson Theophilus Mayo; his granddaughter Susanna Prence, the daughter of his deceased son Thomas; his son-in-law John Freeman; Lydia Sturtevant; and his brother Thomas Clarke.The mention in his will of his deceased son Thomas's daughter Susanna Prence indicates that he died without a surviving male heir in the Prence line.