 Polyimide was used because it adheres to glass well, is chemically stable in aqueous solution, and is biocompatible.It is hypothesized that the microchannels limited the range of angles that cytoskeletal elements within the neurite growth cones could accumulate, assemble, and orient.There was a significant decrease in the number of neurites emerging from the soma; however, there was less decrease as the range of angles over which the neurites emerged was increased.