 It is known that gradients are critical for the guidance of growth cones to their target tissues in the developing nervous system.There has been much research done on soluble gradients; however, little emphasis has been placed on gradients of substratum bound substances of the extracellular matrix such as laminin.Dodla and Bellamkonda, fabricated an anisotropic 3D agarose gel with gradients of coupled laminin-1 (LN-1).