 (2003) developed a scaffold consisting of a single large longitudinally oriented channel was created using chitosan tendons from crabs.Tendons were harvested from crabs (Macrocheira kaempferi) and repeatedly washed with sodium hydroxide solution to remove proteins and to deacetylate the tendon chitin, which subsequently became known as tendon chitosan.A stainless steel bar with triangular-shaped cross-section (each side 2.1 mm long) was inserted into a hollow tendon chitosan tube of circular-shaped cross-section (diameter