 It is important to promote process formation similar to 3D "in vivo" conditions, because astrocytic process morphology is essential in guiding directionality of regenerating axons.The aragonite topography provides a high surface area to volume ratio and lacks edges, which leads to a reduction of the culture edge effect.Crystalline matrices such as the aragonite mentioned here are allowed for the promotion of a complex 3D tissue formation that approaches "in vivo" conditions.