 Over 75% of the AHPCs aligned within 20° of the grooves compared to random growth on the non-patterned substrates.When AHPCs were grown on micropatterned substrates with astrocytes, outgrowth was influenced by the astrocytes that had aligned with the grooves; namely, the AHPCs extended processes along the astrocytic cytoskeletal filaments.However, the alignment was not as significant as that seen by the AHPCs in culture alone with the micropatterned substrate.