 On 10 mg/ml and 1 mg/ml BSA protein patterns and fatty-acid free BSA protein patterns a significant number of neurites avoided the protein lines and grew between the lines.Thus, the fatty-acid-containing BSA lines were just as non-permissive for neurite growth as lines containing BSA with fatty acids.Because microdispensing does not require direct contact with the substrate surfaces, this technique can utilitze surfaces with delicate micro- or nanotopology that could be destroyed by contact.