 He graduated from St John's College, Cambridge as Third Wrangler in 1850 and was elected a fellow of Christ's College in 1852.Collaborating with Percival Frost, a "Treatise on Solid Geometry" was published in 1863.Wolstenholme served as Examiner in 1854, 1856, and 1863 for Cambridge Mathematical Tripos, and according to Andrew Forsyth his book "Mathematical Problems" made a significant contribution to mathematical education