 For submicron particle measurement, dynamic light scattering (DLS) has now become an industry standard technique.This method analyzes the fluctuations of scattered light by particles in suspension when illuminated with a laser to determine the velocity of the Brownian motion, which can then be used to obtain the hydrodynamic size of particles using the Stokes-Einstein relationship.Although DLS is a useful approach to determine the size distribution of many nano and biomaterials systems, it does suffer from several disadvantages.