 Although DLS is a useful approach to determine the size distribution of many nano and biomaterials systems, it does suffer from several disadvantages.For example, DLS is a low-resolution method that is not suitable for measuring polydisperse samples, while the presence of large particles can affect the size accuracy.Other scattering techniques have emerged, such as nanoparticle tracking analysis (NTA), which tracks individual particle movement through scattering using image recording.