 In 1495, John died without surviving legitimate issue and Isabel's brother succeeded him as Manuel I of Portugal.At first, Leanor was made heiress but because she was Dowager Queen and had no surviving children, the post as heiress was passed to Isabel, who in turn passed it to her eldest son Jaime, who was heir from 1495 until 1498 when Manuel's son Miguel da Paz, Prince of Portugal was born.Isabel died in 1521, she was 62 years of age at the Lisbon Convent of the Mother of God, her brother died later in the year, her sister Leonor died 4 years later.