 The western Eurasian haplogroups were observed predominantly and mostly shared in Kho samples with overall frequency of 50%.These include HV8, H19, H57, H24, C and, C4a haplogroups.The South Asian haplogroups and its relevant subgroups including U4, U4c, U6, U5a, and W were also found in Kho samples with overall 37.5% frequency.