 Therefore, the clubs relegated from the Premier League in 2000, Wimbledon, Watford and Sheffield Wednesday, were the top seeds, and the club newly promoted to the Third Division, Kidderminster Harriers, were bottom seeds.The first legs took place on 22 and 23 August and the second legs on 5 and 6 September.The 35 winners from the First Round joined the Premier League clubs not participating in European competition.