 The main advantage of intravital microscopy is that it allows imaging living cells while they are in the true environment of a complex multicellular organism.Thus, intravital microscopy allows researchers to study the behavior of cells in their natural environment or in vivo rather than in a cell culture.Another advantage of intravital microscopy is that the experiment can be set up in a way to allow observing changes in a living tissue of an organism over a period of time.