 Intravital microscopy can be performed using several light microscopy techniques including widefield fluorescence, confocal, multiphoton, spinning disc microscopy and others.The main consideration for the choice of a particular technique is the penetration depth needed to image the area and the amount of cell-cell interaction details required.If the area of interest is located more than 50–100 µm below the surface or there is a need to capture small-scale interactions between cells, multiphoton microscopy is required.