 If the image resolution is high enough (50 – 100 μm) it can be possible to use several images to generate 3D models of cellular interactions, including protrusions that cells make while extending toward each other.3D models from time-lapse image sequences allow assessing speed and directionality of cellular movements.Vascular structures can also be reconstructed in 3D space and changes of their permeability can be monitored throughout a period of time as fluorescent signal intensity of dyes changes when vascular permeability does.