 These included boronate ester formation, diselenides-disulfides exchange, disulphide formation, hemithiolacetal formation, hydrazone formation, imine formation and thiol-enone exchange.For reversible reactions that do not occur in aqueous buffers, the pre-equilibrated DCC approach can be used.The DCL was initially generated (or pre-equilibrated) in organic solvent, and then diluted into aqueous buffer containing the protein target for selection.