 RLC includes Dicer-2 and R2D2, and is crucial to unite Ago2 and RISC.TATA-binding protein-associated factor 11 (TAF11) assembles the RLC by facilitating Dcr-2-R2D2 tetramerization, which increases the binding affinity to siRNA by 10-fold.Association with TAF11 would convert the R2-D2-Initiator (RDI) complex into the RLC.