 In eukaryotes, it is first acylated on its "sn"-1 position by an ER- or mitochondrial membrane enzyme, glycerol-3-phosphate O-acyltransferase, and another acyl group is then added on the "sn"-2 position making phosphatidic acids.Glycerol-1-phosphatase removes the phosphate group of glycerol 3-phosphate to generate glycerol, allowing glycerol fermentation to produce glycerol from glucose through glycolysis pathway.A number of microbes, plants and mammals have been shown to express this enzyme.