 Fane was educated at Maidstone Grammar School in Kent and in about 1595 matriculated at Queens' College, Cambridge.He was admitted to Lincoln's Inn on 19 November 1597, for training as a lawyer.In 1601, with the support of his near neighbour Henry Brooke, 11th Baron Cobham, lord of the Manor of Cobham, Kent, Fane was returned as a Member of Parliament for Kent.