 Dipeptidases are secreted onto the brush border of the villi in the small intestine, where they cleave dipeptides into their two component amino acids prior to absorption.DIPEPTIDASES convert dipeptides into amino acids

Dipeptidases are also found within the enterocytes themselves, performing cytosolic digestion of absorbed dipeptides.They are exopeptidases, classified under EC number 3.4.13.