 Tuke became Principal of Bedford College in 1907 and remained in the post until her retirement in 1929.During her time there she oversaw its move from Baker Street, London, to a site at Regent's Park, after which the number of students doubled.While overseeing the expansion of the academic provisions at the College, she also served as the representative of the Faculty of Arts on the University of London Senate from 1911 until her retirement.