 Serbelloni entered the ranks of Knights of Malta, sending troops to help break the Turkish siege and organizing naval raids against the Muslims.After receiving the title of Prior of Hungary, Serbelloni oversaw the reconstruction of the Maltese fortifications damaged or destroyed under the Turkish assault, and acted as middle-man between Jean de la Vallette, his order's Grand Master, and the viceroy of Sicily, don Garcia de Toledo.In 1567, Serbelloni followed the Spanish armies into Belgium under the Duke of Alva during the repression of the Dutch Revolt, being appointed twice as governor of Antwerp.