 Like first-generation Glycoazodyes, second-generation Glycoazodyes are synthesized using a glucose, galactose, or lactose sugar group.The point of the ether bond is controlled by selectively protecting alcohol groups on the sugar, or by choosing an azo dye with a different alcohol group position.An unprotected alcohol group of either the sugar or the dye is reacted with an n-carbon, terminal dibromoalkane in a solution of potassium hydroxide and 18-crown-6 ether, using non-anhydrous tetrahydrofuran as the solvent.