 Hence, all Y chromosomes belonging to DE, D, E and their subclades are YAP-positive (YAP+).All Y chromosomes that belong to other haplogroups and subclades are YAP-negative (YAP-).The age of haplogroup DE, previously estimated at between 65,000 and 71,000 years, was later estimated at around 73,000 years  and most recently at around 76,000 years old.