 Investigation of the n-hexane and EtOAc fractions of the root extract of "P. persica" using bioassay-guided isolation techniques led to the isolation of ent-epiafzelechin-(2α→O→7’,4α→8’)-(-)ent-afzelechin (1, "S. aureus" MIC 156 µg/mL, MRSA and MDRSA MIC 312 µg/mL, "E. coli" β-, "S. typhimurium" and "P. aeruginosa" MIC 2500 µg/mL), afzelechin (2, not active), α-cyanobenzyl benzoate (3, "S. aureus", MDRSA and MRSA 78 µg/mL, "E. coli" β- MIC 312 µg/mL and "P. aeruginosa" MIC 625 µg/mL), β-sitosterol (4, S. aureus, MDRSA, MRSA and "E. coli" β- MIC 2500 µg/mL, "S. typhimurium" MIC 625 µg/mL, "P. aeruginosa" MIC 1250 µg/mL) and stigmast-4-en-3-one (5, "S. aureus", MDRSA and MRSA MIC 156 µg/mL, "E. coli" β- MIC 312, "S. typhimurium" MIC 625 µg/mL and "P. aeruginosa" MIC 1250 µg/mL).Very good antimicrobial activity of the isolated compounds 1 and 3 and good activity of compound 4 against a range of dermatologically relevant bacteria supports the traditional application of this plant to treat skin related ailments.This is the first report of the isolated compounds 1 and 3 possessing antimicrobial activities as well as the first report of ent-epiafzelechin-(2α→O→7’,4α→8’)-(-)ent-afzelechin and α-cyanobenzyl benzoate being isolated from genus Prunus.