 Ascorbate is a known cofactor of myrosinase, serving as a base catalyst in glucosinolate hydrolysis.For example, myrosinase isolated from daikon ("Raphanus sativus") demonstrated an increase in V max from 2.06 μmol/min per mg of protein to 280 μmol/min per mg of protein on the substrate, allyl glucosinolate (sinigrin) when in the presence of 500 μM ascorbate.Sulfate, a byproduct of glucosinolate hydrolysis, has been identified as a competitive inhibitor of myrosinase.