 Myrosinase is regarded as a defense-related enzyme and is capable of hydrolyzing glucosinolates into various compounds, some of which are toxic.Myrosinase catalyzes the chemical reaction

Thus, the two substrates of this enzyme are thioglucoside and H2O, whereas its two products are sugar and thiol.In the presence of water, myrosinase cleaves off the glucose group from a glucosinolate.