 Borate and a suitable polymer bed are used to chromatograph non-glycosylated hemoglobin differentially from glycosylated hemoglobin (chiefly HbA1c), which is an indicator of long-term hyperglycemia in diabetes mellitus.Borax alone does not have a high affinity for the "hardness cations", although it has been used for water-softening.Its chemical equation for "water-softening" is given below