 Taking the cell culture example, a hyperspectral image could show the distribution of cholesterol, as well as proteins, nucleic acids, and fatty acids.Sophisticated signal- and image-processing techniques can be used to ignore the presence of water, culture media, buffers, and other interferences.Because a Raman microscope is a diffraction-limited system, its spatial resolution depends on the wavelength of light, the numerical aperture of the focusing element, and — in the case of confocal microscopy — on the diameter of the confocal aperture.