 In the early 1900s, an observation was made, showing an accumulation of free fatty acids after incubation of pancreatic juice with phosphatidylcholine.One of the first cases of observed PLA1 activity was on 1903 when snake venom was found to alter phosphatidylcholine into lysophosphatidylcholine, which is defined as a phosphatidylcholine without one of its fatty acids.In the 1960s, it was discovered to be that enzymes catalyze this fatty acid cleavage in multiple ways, one of which is the sn-1 position.