 Protein threading, also known as fold recognition or 3D-1D alignment, can also be used as a search technique for identifying templates to be used in traditional homology modeling methods.Recent CASP experiments indicate that some protein threading methods such as RaptorX indeed are more sensitive than purely sequence(profile)-based methods when only distantly-related templates are available for the proteins under prediction.When performing a BLAST search, a reliable first approach is to identify hits with a sufficiently low "E"-value, which are considered sufficiently close in evolution to make a reliable homology model.