 First, a portion of the infected tissue will be surgically removed and used for a biopsy.Since the fungus can not tolerate refrigeration, the biopsied material needs to be incubated immediately once it is collected.Then, the examination will investigate the presence of thin-walled, wide, hyaline, coenocytic hyphae and internal cleavage for the production of the sporangiospores in H&E (Haemotoxylin and Eosin) stained sections.