 He entered the most prestigious of Roman seminaries, the Pontifical Roman Seminary, and after becoming a priest in 1908 soon took up a role as an official in the vicariate of Rome.Despite doing some work as a pastor in Rome, di Jorio was always chiefly concerned with his work in the Vatican bureaucracy, and in 1918 he took up a role as president of the "Istituto per le Opere di Religione" ("Institute of Religious Works").Under the direction of Pope Pius XI, who was eager to settle the "Roman Question" that had kept the Pope as a "prisoner of the Vatican" since Italian unification in 1870, di Jorio formed a close association with Bernardino Nogara in the 1920s.