 However, due to their difficulty to be cultivated in laboratory settings, the entire genome was only sequenced in one species, "Nitrospira defluvii".In addition, "Nitrospira" bacteria's 16s rRNA sequences are too dissimilar to use for PCR primers, thus some members go unnoticed.In addition, members of Nitrospira with the capabilities to perform complete nitrification (Comammox bacteria) has also been discovered.