 Because transamination reactions are readily reversible and pyruvate is present in all cells, alanine can be easily formed and thus has close links to metabolic pathways such as glycolysis, gluconeogenesis, and the citric acid cycle.L-Alanine is produced industrially by decarboxylation of L-aspartate by the action of aspartate 4-decarboxylase.Fermentation routes to L-alanine are complicated by alanine racemase.