 The route on Hamilton Avenue was not one of the original franchises granted to the Brooklyn City Rail Road in 1853, but was added soon after.The portion south of Court Street, which was one of the original routes, opened on September 4, 1854 as part of the Court Street Line to Greenwood Cemetery, and the rest opened in early June 1855, at first operated as a shuttle between the ferry and Court Street.Eventually the route was operated along Third Avenue south to the 65th Street Depot in Bay Ridge as the 33 Hamilton line.