 The positively charged nitrogen created by this interaction may then interact with the deprotonated glutamate, causing a shift in ring orientation and exposing the methyl group derived from the methylamine to the binding cleft where it can interact with corrinoid.In this way a net is transferred to the cofactor's cobalt atom with a change of oxidation state from I to III.The methylamine-derived ammonia is then released, restoring the original imine.