 He sat in parliament as bishop, and was challenged by the synod of Fife, meeting at St. Andrews 3 Feb. 1601, for doing so, when he declared he was obliged to answer ‘with the name of Bishop put against his will, because they would not name him otherwise’.Gladstanes continued to be minister of St. Andrews.He was employed by the assembly on various commissions for dealing with the papists, for the plantation of kirks, and for visiting presbyteries.