 State Route 301, which had not existed immediately prior to 1940, was assigned as a placeholder for the extension of U.S. Route 301 into Virginia, following U.S. Route 1 from Petersburg to Richmond, State Route 2 from Richmond to Bowling Green, and State Route 207 from Bowling Green to Maryland to match Maryland Route 301.State Route 305, from Damascus to Mouth of Wilson, was eliminated and transferred to a rerouted US 58.State Route 320, which had not existed immediately prior to 1940, was assigned as a renumbering of State Route 123.