 In the night between 21 and 22 July 1444 he was killed by conspirators in the Ducal Palace, together with his counsellors Manfredo dei Pio da Carpi and Tommaso di Guido dell'Agnello.Federico succeeded him as Duke of Urbino.Questions have been raised about the possibility of his involvement in Oddantonio's assassination, though no definitive proof of any such involvement exists.