 He studied law, was admitted to the bar in 1833, and commenced practice in Easton, Maryland.He served as a member of the Maryland House of Delegates in 1834 and 1835, and was State's attorney for Talbot County from 1836 to 1844.Hambleton served in the Maryland State Senate from 1844 to 1850, was president of the Chesapeake & Ohio Canal in 1853 and 1854, and again served as a member of the House of Delegates in 1853.