 Her second marriage was to the Spanish ambassador, Juan de Silva, very influential during the reign of Sebastian I of Portugal and who, on his marriage, became 4th Count of Portalegre and inherited the office of Mordomo-mór.He participated with the Portuguese in the Battle of Alcacer Quibir, where he fell prisoner to the moors.During the transitional reign of Henry I of Portugal, the Cardinal King, he was a firm supporter of Philip II of Spain's claims to the throne of Portugal.