 She made her professional opera debut on 18 April 1987 at the Divadlo Josefa Kajetána Tyla in Plzeň, remaining at that theatre for the next three years.Among the roles she portrayed at that house include Amelia in Giuseppe Verdi's "Un ballo in maschera", Julie in Antonín Dvořák's "The Jacobin", and Milada in Bedřich Smetana's "Dalibor".Urbanová made her debut at the National Theater in Prague on 16 March 1990 as Milada under the baton of conductor Zdeněk Košler.