 Here, the duration of cell cycle arrest depends not only on the final level of input Fus3, but also on the previously achieved Fus3 levels.This effect is achieved due to the slower time scales involved in the transcription of intermediate Far1, such that the total Far1 activity reaches its equilibrium value slowly, and for transient changes in Fus3 concentration, the response of the system depends on the Far1 concentration achieved with the transient value.Experiments in this type of hysteresis benefit from the ability to change the concentration of the inputs with time.