 After computing the translational displacement field between a deformed and undeformed configuration, a strain field can be computed.From the strain field, the stress field surrounding the cell can be calculated with knowledge of the stress-strain behavior, or constitutive model, of the surrounding hydrogel material.It is possible to proceed one step further, and use the stress field to compute the tractions at the surface of the cell, if the normal vectors to the cell surface can be obtained from a 3D image stack.