 Recent advances in TFM have shown that cells are capable of exerting deformations with strain magnitudes up to 40%, which requires usage of a finite deformation theory approach to account for large strain magnitudes.Although TFM is frequently used to observe tractions at the surface of a spatially isolated individual cell, a variation of TFM can also be used to analyze the collective behavior of multicellular systems.For example, cellular migration velocities and plithotaxis are observed alongside a computed stress variation map of a monolayer sheet of cells, in an approach termed monolayer stress microscopy.