 The immobilization should occur in the morning when temperatures are cooler, the immobilized animals' ears and eyes should be covered to reduce stress while sampling, measurements and other procedures occur, after which an antidote is administered in order to reverse the tranquilization effect.Cat species are particularly vulnerable to anesthesia and should be monitored for 24 hours after immobilization.Criticism has sprung up over the possibility that particular animals may undergo tranquilization too frequently in the interest of generating revenue, with cases of corruption culminating in a loss of support for green hunting by government, conservation and hunting organizations.