 Cane uses C4 carbon fixation, and beet uses C3 carbon fixation, resulting in a different ratio of 13C and 12C isotopes in the sucrose.Tests are used to detect fraudulent abuse of European Union subsidies or to aid in the detection of adulterated fruit juice.Sugar cane tolerates hot climates better, but the production of sugar cane needs approximately four times as much water as the production of sugar beet.